By M. Cristina Vega
This ebook offers complicated expression applied sciences for the construction of protein complexes. considering the fact that complexes lie on the center of recent biology, the expression, purification, and characterization of huge quantities of top quality protein complexes is essential for the fields of biomedicine, biotechnology, and structural biology. From co-expression in E. coli, yeast, mammalian and bug cells to advanced reconstitution from person subunits, this publication bargains invaluable insights and assistance for winning protein expressionists.
Across numerous sections readers will detect present possibilities for the construction of protein complexes in bacterial structures (including membrane proteins and cell-free co-expression), methylotrophic and non-methylotrophic yeasts, protozoa (Leishmania terantolae and Dictyostelium discoideum), baculovirus-infected insect cells, mammalian cells, crops and algae. advanced reconstitution from separately purified subunits or subcomplexes is mentioned as a complementary process. a final part introduces in brief many of the biophysical and structural characterization suggestions for macromolecular complexes utilizing cutting-edge resolution scattering and nuclear magnetic resonance.
This paintings is a guided travel over probably the most robust and profitable protein expression applied sciences, with a spotlight on co-expression and high-throughput purposes. it's addressed to every body drawn to the construction and characterization of macromolecular complexes, from college scholars who wish an available description of the key co-expression platforms to researchers in biomedicine and the lifestyles sciences looking for an updated survey of obtainable technologies.
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Extra info for Advanced Technologies for Protein Complex Production and Characterization (Advances in Experimental Medicine and Biology)
Advanced Technologies for Protein Complex Production and Characterization (Advances in Experimental Medicine and Biology) by M. Cristina Vega